Lambda Light Chain Antibody / Lambda Antibody

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NSJ Bioreagents
Product Code: NSJ-R20161BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20161BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM110
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse Lambda Light Chain antibody at -20oC.

Images

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Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa; and IgG1 lambda; (20ng/lane), using 0.2ug/ml of recombinant Mouse Lambda Light Chain antibody (clone RM110). This mAb reacts to nonreduced IgG1 lambda;.
2 / 3
ELISA of mouse immunoglobulins shows recombinant Mouse Lambda Light Chain antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa, human IgG, rat IgG, or goat IgG.
3 / 3
A titer ELISA of mouse IgG1 lambda;. The plate was coated with different amounts of mouse IgG1 lambda;. A serial dilution of recombinant Mouse Lambda Light Chain antibody was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa; and IgG1 lambda; (20ng/lane), using 0.2ug/ml of recombinant Mouse Lambda Light Chain antibody (clone RM110). This mAb reacts to nonreduced IgG1 lambda;.
ELISA of mouse immunoglobulins shows recombinant Mouse Lambda Light Chain antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa, human IgG, rat IgG, or goat IgG.
A titer ELISA of mouse IgG1 lambda;. The plate was coated with different amounts of mouse IgG1 lambda;. A serial dilution of recombinant Mouse Lambda Light Chain antibody was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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Further Information

Application Details:
ELISA: 0.005-0.2ug/ml,Western Blot (non-reduced): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse Lambda Light Chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse Lambda Light Chain antibody reacts to the lamda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgM Lambda was used as the immunogen for this recombinant Mouse Lambda Light Chain antibody.
Limitation:
This recombinant Mouse Lambda Light Chain antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01843