Mouse IgG2b Antibody

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NSJ Bioreagents
Product Code: NSJ-R20167BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20167BTN-50UG50ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM108
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG2b antibody at -20oC.

Images

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Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2b antibody reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
3 / 3
ELISA titer: the plate was coated with different amounts of mouse IgG2b. A serial dilution of recombinant Mouse IgG2b antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2b antibody reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
ELISA titer: the plate was coated with different amounts of mouse IgG2b. A serial dilution of recombinant Mouse IgG2b antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.005ug/ml - 0.2ug/ml,Western Blot (stronger on non-reduced samples): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2b antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG2b antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG2b was used as the immunogen for this recombinant Mouse IgG2b antibody.
Limitation:
This recombinant Mouse IgG2b antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01867