Mouse IgG2b Antibody

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NSJ Bioreagents
Product Code: NSJ-R20167
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20167-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM108
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG2b antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 3
Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
2 / 3
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2b antibody reacts to the Fc region of mIgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human/rat/goat IgG.
3 / 3
ELISA titer: the plate was coated with different amounts of mouse IgG2b. A serial dilution of recombinant Mouse IgG2b antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2b antibody reacts to the Fc region of mIgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human/rat/goat IgG.
ELISA titer: the plate was coated with different amounts of mouse IgG2b. A serial dilution of recombinant Mouse IgG2b antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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Further Information

Application Details:
ELISA: 0.005ug/ml - 0.2ug/ml,Western Blot (stronger on non-reduced samples): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2b antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG2b antibody reacts to the Fc region of mIgG2b. No cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG2b was used as the immunogen for this recombinant Mouse IgG2b antibody.
Limitation:
This recombinant Mouse IgG2b antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01867