IgG2a-Kappa Antibody

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NSJ Bioreagents
Product Code: NSJ-R20165BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20165BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM107
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG2a-Kappa antibody at -20oC.

Images

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Western blot of nonreduced(-) and reduced(+) mouse IgG2a kappa; (20 ng/lane), using 0.2ug/ml of recombinant Mouse IgG2a-Kappa antibody. This antibody only reacts to nonreduced Mouse IgG2a kappa;.
2 / 3
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mouse IgG2a kappa;; no cross reactivity with IgG2a-lambda, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
3 / 3
A titer ELISA of mouse IgG2a, kappa;. The plate was coated with different amounts of mouse IgG2a kappa;. A serial dilution of recombinant Mouse IgG2a-Kappa antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgG2a kappa; (20 ng/lane), using 0.2ug/ml of recombinant Mouse IgG2a-Kappa antibody. This antibody only reacts to nonreduced Mouse IgG2a kappa;.
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mouse IgG2a kappa;; no cross reactivity with IgG2a-lambda, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
A titer ELISA of mouse IgG2a, kappa;. The plate was coated with different amounts of mouse IgG2a kappa;. A serial dilution of recombinant Mouse IgG2a-Kappa antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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Further Information

Application Details:
ELISA: 0.005ug/ml - 0.2ug/ml,Western Blot (non-reduced only): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2a-Kappa antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mouse IgG2ak. No cross reactivity with mouse IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG was used as the immunogen for this recombinant Mouse IgG2a-Kappa antibody.
Limitation:
This recombinant Mouse IgG2a-Kappa antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01863