Mouse IgG1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R20164BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20164BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM106
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG1 antibody at -20oC.

Images

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Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of recombinant Mouse IgG1 antibody. This mAb reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
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ELISA of mouse immunoglobulins shows recombinant Mouse IgG1 antibody reacts to the Fc region of mouse IgG1; slightly cross reacts to IgG2b; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
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ELISA titer: the plate was coated with different amount of mouse IgG1. A serial dilution of recombinant Mouse IgG1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of recombinant Mouse IgG1 antibody. This mAb reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
ELISA of mouse immunoglobulins shows recombinant Mouse IgG1 antibody reacts to the Fc region of mouse IgG1; slightly cross reacts to IgG2b; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
ELISA titer: the plate was coated with different amount of mouse IgG1. A serial dilution of recombinant Mouse IgG1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.005-0.2ug/ml,Western Blot: 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG1 antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG was used as the immunogen for this recombinant Mouse IgG1 antibody.
Limitation:
This recombinant Mouse IgG1 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01868