IgG Heavy Chain Antibody

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NSJ Bioreagents
Product Code: NSJ-R20163
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20163-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM104
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20ng/lane), using 0.2ug/ml of recombinant Mouse IgG antibody. This mAb reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG antibody reacts to the Fc region of mIgG1, mIgG2a, mIgG2b, and mIgG3; no cross reactivity with IgM, IgA, IgE, human/rat IgG.
3 / 3
ELISA titer: the plate was coated with different amounts of mouse IgG. A serial dilution of recombinant Mouse IgG antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20ng/lane), using 0.2ug/ml of recombinant Mouse IgG antibody. This mAb reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG antibody reacts to the Fc region of mIgG1, mIgG2a, mIgG2b, and mIgG3; no cross reactivity with IgM, IgA, IgE, human/rat IgG.
ELISA titer: the plate was coated with different amounts of mouse IgG. A serial dilution of recombinant Mouse IgG antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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Further Information

Application Details:
ELISA: 0.005-0.2ug/ml,Western Blot (non-reduced): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human/rat IgG. It may cross react to goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG was used as the immunogen for this recombinant Mouse IgG antibody.
Limitation:
This recombinant Mouse IgG antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01868