Mouse Kappa Light Chain Antibody

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NSJ Bioreagents
Product Code: NSJ-R20162BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20162BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM103
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse Kappa Light Chain antibody at -20oC.

Images

1 / 3
ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG ( kappa;+ lambda;), rat IgG ( kappa;+ lambda;), or goat IgG ( kappa;+ lambda;).
2 / 3
Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa; and IgG1 lambda; (20ng/lane), using 0.2ug/ml of recombinant Mouse Kappa Light Chain antibody. This mAb reacts to nonreduced IgG1 kappa; (~150 kDa), and slightly reacts to reduced kappa; light chain (~25 kDa).
3 / 3
A titer ELISA of mouse IgG1 kappa;. The plate was coated with different amounts of mouse IgG1 kappa;. A serial dilution of recombinant Mouse Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as secondary.
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ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG ( kappa;+ lambda;), rat IgG ( kappa;+ lambda;), or goat IgG ( kappa;+ lambda;).
Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa; and IgG1 lambda; (20ng/lane), using 0.2ug/ml of recombinant Mouse Kappa Light Chain antibody. This mAb reacts to nonreduced IgG1 kappa; (~150 kDa), and slightly reacts to reduced kappa; light chain (~25 kDa).
A titer ELISA of mouse IgG1 kappa;. The plate was coated with different amounts of mouse IgG1 kappa;. A serial dilution of recombinant Mouse Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as secondary.

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Further Information

Application Details:
ELISA: 0.005-0.2ug/ml,Western Blot (non-reduced): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse Kappa Light Chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG was used as the immunogen for this recombinant Mouse Kappa Light Chain antibody.
Limitation:
This recombinant Mouse Kappa Light Chain antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01837