Mouse Kappa Light Chain Antibody

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NSJ Bioreagents
Product Code: NSJ-R20162
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20162-100UG100ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM103
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse Kappa Light Chain antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa; and IgG1 lambda; (20ng/lane), using 0.2ug/mL of recombinant Mouse Kappa Light Chain antibody. This mAb reacts to nonreduced IgG1 kappa; (~150 kDa), and slightly reacts to reduced kappa; light chain (~25 kDa).
2 / 3
ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mIgs. No cross reactivity with the lambda light chain or human/rat/goat IgG ( kappa;+ lambda;).
3 / 3
ELISA titer: the plate was coated with different amounts of mouse IgG1 kappa;. A serial dilution of recombinant Mouse Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa; and IgG1 lambda; (20ng/lane), using 0.2ug/mL of recombinant Mouse Kappa Light Chain antibody. This mAb reacts to nonreduced IgG1 kappa; (~150 kDa), and slightly reacts to reduced kappa; light chain (~25 kDa).
ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mIgs. No cross reactivity with the lambda light chain or human/rat/goat IgG ( kappa;+ lambda;).
ELISA titer: the plate was coated with different amounts of mouse IgG1 kappa;. A serial dilution of recombinant Mouse Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as secondary.

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Further Information

Application Details:
ELISA: 0.005-0.2ug/ml,Western Blot (non-reduced): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse Kappa Light Chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of murine immunoglobulins. No cross reactivity with the lamda chain or human/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG was used as the immunogen for this recombinant Mouse Kappa Light Chain antibody.
Limitation:
This recombinant Mouse Kappa Light Chain antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01837