Phosphothreonine Antibody

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NSJ Bioreagents
Product Code: NSJ-R20193
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20193-100UG100ug£581.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM102
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Phosphothreonine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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ICC of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:500 (PE-secondary, red) and DAPI (blue).
2 / 4
Western blot of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:2000.
3 / 4
IP of Calyculin A/Okadaic Acid treated human A431 cells by the recombinant Phosphothreonine antibody at 1:500, and then blotted with same mAb at 1:2000. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.
4 / 4
The recombinant Phosphothreonine antibody recognizes phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.
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ICC of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:500 (PE-secondary, red) and DAPI (blue).
Western blot of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:2000.
IP of Calyculin A/Okadaic Acid treated human A431 cells by the recombinant Phosphothreonine antibody at 1:500, and then blotted with same mAb at 1:2000. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.
The recombinant Phosphothreonine antibody recognizes phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.

Documents

Further Information

Application Details:
Western blot: 1:500 -1:2000 dilution,Immunoprecipitation: 1:100-1:500 dilution,Chromatin IP (ChIP): 1:100-1:500 dilution,Immunocytochemistry: 1:100-1:500 dilution,Immunohistochemistry: 1:100-1:500 dilution (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Phosphothreonine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Phosphothreonine antibody reacts with threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide was used as the immunogen for this recombinant Phosphothreonine antibody.
Limitation:
This recombinant Phosphothreonine antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant