Acetyl Lysine Antibody

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NSJ Bioreagents
Product Code: NSJ-R20192
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20192-100UG100ug£558.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM101
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Acetyl Lysine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 5
ICC staining of human HeLa cells using recombinant Acetyl Lysine antibody (red). Actin filaments was labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
2 / 5
Western blot of human HeLa cells non-treated or treated with Trichostatin A (TSA) with recombinant Acetyl Lysine antibody at 1:2000.
3 / 5
IP of human A431 cell lysate with recombinant Acetyl Lysine antibody at 1:500 and probed with anti-PTEN mouse mAb.
4 / 5
The recombinant Acetyl Lysine antibody recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.
5 / 5
The recombinant Acetyl Lysine antibody recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.
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ICC staining of human HeLa cells using recombinant Acetyl Lysine antibody (red). Actin filaments was labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
Western blot of human HeLa cells non-treated or treated with Trichostatin A (TSA) with recombinant Acetyl Lysine antibody at 1:2000.
IP of human A431 cell lysate with recombinant Acetyl Lysine antibody at 1:500 and probed with anti-PTEN mouse mAb.
The recombinant Acetyl Lysine antibody recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.
The recombinant Acetyl Lysine antibody recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.

Documents

Further Information

Application Details:
Western blot: 1:500 -1:2000 dilution,Immunoprecipitation: 1:100-1:500 dilution,Chromatin IP (ChIP): 1:100-1:500 dilution,Immunocytochemistry: 1:100-1:500 dilution,Immunohistochemistry: 1:100-1:500 dilution (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Acetyl Lysine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Acetyl Lysine antibody reacts to lysine-acetylated proteins. No cross reactivity with non-acetylated lysine, and lysine with other modification.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Acetyllysine-BSA was used as the immunogen for this recombinant Acetyl Lysine antibody.
Limitation:
This recombinant Acetyl Lysine antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant