Cytokeratin 18 Antibody

NSJ Bioreagents
Product Code: NSJ-V7922SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7922SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rKRT18/1190
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human colon carcinoma with recombinant Cytokeratin 18 antibody (clone rKRT18/1190). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human colon carcinoma with recombinant Cytokeratin 18 antibody (clone rKRT18/1190). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of human samples with recombinant Cytokeratin 18 antibody. Predicted molecular weight ~48 kDa.

IHC staining of FFPE human colon carcinoma with recombinant Cytokeratin 18 antibody (clone rKRT18/1190). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant Cytokeratin 18 antibody (clone rKRT18/1190). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human samples with recombinant Cytokeratin 18 antibody. Predicted molecular weight ~48 kDa.

Documents

Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human KRT18 protein was used as the immunogen for the recombinant Cytokeratin 18 antibody.
Limitation:
This recombinant Cytokeratin 18 antibody is available for research use only.
Purity:
Protein G affinity chromatography