KRT10 Antibody / Cytokeratin 10

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NSJ Bioreagents
Product Code: NSJ-V3603SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3603SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rKRT10/844
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant KRT10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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IHC testing of FFPE human skin with recombinant KRT10 antibody (clone rKRT10/844). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human skin with recombinant KRT10 antibody (clone rKRT10/844). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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Further Information

Application Details:
Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant KRT10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (KRT10). KRT10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of KRT10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, KRT10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), KRT10 is detected very frequently. Expression is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human Cytokeratin 10 protein was used as the immunogen for the recombinant KRT10 antibody.
Limitation:
This recombinant KRT10 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P13645