EpCAM Antibody / Extracellular domain

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NSJ Bioreagents
Product Code: NSJ-V3570IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3570IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rEGP40/1372
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human skin with recombinant EpCAM antibody (clone rEPG40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human rectal mass tissue with recombinant EpCAM antibody (clone rEPG40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC staining of FFPE cat small intestine with recombinant EpCAM antibody (clone rEGP40/1372). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant EpCAM antibody (clone rEGP40/1372). These results demonstrate the foremost specificity of the rEGP40/1372 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC testing of FFPE human skin with recombinant EpCAM antibody (clone rEPG40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human rectal mass tissue with recombinant EpCAM antibody (clone rEPG40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE cat small intestine with recombinant EpCAM antibody (clone rEGP40/1372). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant EpCAM antibody (clone rEGP40/1372). These results demonstrate the foremost specificity of the rEGP40/1372 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
Limitation:
This recombinant EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P16422