CD81 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8525SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8525SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rC81/3442
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Functional Study
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant CD811 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry staining of human MCF7 cells with recombinant CD81 antibody; Gray=unstained cells, Orange= recombinant CD81 antibody.
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Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone rC81/3442). Gray=unstained cells, Orange = recombinant CD81 antibody.
3 / 4
IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Flow cytometry staining of human MCF7 cells with recombinant CD81 antibody; Gray=unstained cells, Orange= recombinant CD81 antibody.
Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone rC81/3442). Gray=unstained cells, Orange = recombinant CD81 antibody.
IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Functional Studies: order Ab format without BSA/sodium azide,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 1-2ug/ml for 2 hours at RT,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.
Description:
Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human CD81 protein was used as the immunogen for the recombinant CD81 antibody.
Limitation:
This recombinant CD81 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity chromatography
Uniprot #:
P60033