BrdU Antibody / Bromodeoxyuridine

NSJ Bioreagents
Product Code: NSJ-V8369SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8369SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rBRD494
Regulatory Status: RUO
Target Species: Species Independent
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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SDS-PAGE analysis of purified, BSA-free recombinant BrdU antibody (clone rBRD494) as confirmation of integrity and purity.

SDS-PAGE analysis of purified, BSA-free recombinant BrdU antibody (clone rBRD494) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.
Description:
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.
Limitation:
This recombinant BrdU antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography