Retinol Binding Protein 4 Antibody / RBP4

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NSJ Bioreagents
Product Code: NSJ-V8704SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8704SAF-100UG100 ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: RBP4/4051
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the RBP4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human kidney with RBP4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human kidney with RBP4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RBP4 antibody. These results demonstrate the foremost specificity of the RBP4/4051 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human kidney with RBP4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with RBP4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RBP4 antibody. These results demonstrate the foremost specificity of the RBP4/4051 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the RBP4 antibody should be determined by the researcher.
Description:
Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds diagnostic importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal Vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP, resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with Insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 29-148 from the human protein was used as the immunogen for the RBP4 antibody.
Limitation:
This RBP4 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P02753