Bcl-2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3543IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3543IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rBCL2/796
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (rBCL2/796). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.~
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SDS-PAGE analysis of purified, B
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IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (rBCL2/796). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.~
SDS-PAGE analysis of purified, B

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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant full-length human protein was used as the immunogen for the recombinant Bcl-2 antibody.
Limitation:
This recombinant Bcl-2 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P10415