Bcl-2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3543SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3543SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rBCL2/796
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Bcl-2 antibody (clone rBCL2/796). These results demonstrate the foremost specificity of the rBCL2/796 mAb. <BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (rBCL2/796). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Western blot testing of human samples with recombinant Bcl-2 antibody (clone rBCL2/796). Expected molecular weight ~26 kDa.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Bcl-2 antibody (clone rBCL2/796). These results demonstrate the foremost specificity of the rBCL2/796 mAb. <BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (rBCL2/796). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human samples with recombinant Bcl-2 antibody (clone rBCL2/796). Expected molecular weight ~26 kDa.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the recombinant Bcl-2 antibody.
Limitation:
This recombinant Bcl-2 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P10415