Bcl-2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3546IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3546IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rBCL2/782
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Bcl2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of human follicular lymphoma with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.~
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SDS-PAGE analysis of purified, BSA-free recombinant B
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IHC testing of human follicular lymphoma with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.~
SDS-PAGE analysis of purified, BSA-free recombinant B

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the recombinant Bcl2 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant full-length human protein was used as the immunogen for the recombinant Bcl2 antibody.
Limitation:
This recombinant Bcl2 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P10415