CD34 Antibody Cocktail
| Code | Size | Price |
|---|
| NSJ-V2999SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 lambda
Antibody Clonality: Monoclonal
Antibody Clone: QBEnd/10 + HPCA1/763
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the CD34 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD34 antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10) and recombinant full-length human protein (HPCA1/763) were used as the immunogen for the CD34 antibody cocktail.
Limitation:
This CD34 antibody cocktail is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P28906