CD45 Antibody (Leukocyte marker)

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NSJ Bioreagents
Product Code: NSJ-V3656SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3656SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: PTPRC/1975R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The recombinant CD45 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

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IHC testing of recombinant CD45 antibody and FFPE human lymph node (clone PTPRC/1975R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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Flow cytometry testing of PFA-fixed human Raji cells with CD45 antibody (clone PTPRC/1975R); Red=isotype control, Blue= CD45 antibody.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant CD45 antibody (clone PTPRC/1975R) as confirmation of integrity and purity.
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IHC testing of recombinant CD45 antibody and FFPE human lymph node (clone PTPRC/1975R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of PFA-fixed human Raji cells with CD45 antibody (clone PTPRC/1975R); Red=isotype control, Blue= CD45 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant CD45 antibody (clone PTPRC/1975R) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Due to variation in protocol and secondary antibody used, the recombinant CD45 antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as immunogen for this recombinant CD45 antibody.
Limitation:
This recombinant CD45 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P08575