S100A4 Antibody / FSP1

NSJ Bioreagents
Product Code: NSJ-V7339SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7339SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: PS1A4-1R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody (clone PS1A4-1R). Predicted molecular weight ~12 kDa.~
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IHC testing of FFPE human placenta with recombinant S100A4 antibody (clone PS1A4-1R). Required HIER: s

Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody (clone PS1A4-1R). Predicted molecular weight ~12 kDa.~
IHC testing of FFPE human placenta with recombinant S100A4 antibody (clone PS1A4-1R). Required HIER: s

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the S100A4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A4 may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [RefSeq]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.
Limitation:
This recombinant S100A4 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein A affinity chromatography
Uniprot #:
P26447