Progesterone Receptor Antibody
Code | Size | Price |
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NSJ-V2224SAF-100UG | 100 ug | £534.00 |
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: PR484
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Progesterone Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Progesterone Receptor antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone. This progesterone receptor antibody is excellent for immunohistochemical staining of formalin/paraffin tissues.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
5241
Immunogen:
Recombinant human protein was used as the immunogen for this Progesterone Receptor antibody.
Limitation:
This Progesterone Receptor antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity chromatography