Galectin 13 Antibody / LGALS13

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NSJ Bioreagents
Product Code: NSJ-V3295SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3295SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: PP13/1161
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Galectin 13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human placenta with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.
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IHC testing of FFPE human spleen with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.
3 / 3
SDS-PAGE analysis of purified, BSA-free Galectin 13 antibody (clone PP13/1161) as confirmation of integrity and purity.
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IHC testing of FFPE human placenta with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.
IHC testing of FFPE human spleen with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.
SDS-PAGE analysis of purified, BSA-free Galectin 13 antibody (clone PP13/1161) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Titering of the Galectin 13 antibody may be required for optimal performance.
Description:
It recognizes a 32kDa protein, which is identified as homodimer of LGALS13/Galectin 13 (also known as PP13). Galectins are a family of soluble beta-galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, Galectin 13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, Galectin-13 exists as a disulfide-linked homodimer. Galectin 13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-maternal blood-spaces. Screening for Galectin 13 in maternal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A human partial recombinant protein corresponding to amino acids 23-134 was used as the immunogen for the Galectin 13 antibody.
Limitation:
This Galectin 13 antibody is available for research use only.
Localization:
Cytoplasmic, cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
Q9UHV8