GCDFP-15 Antibody / PIP / Prolactin-Induced Protein

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NSJ Bioreagents
Product Code: NSJ-V3281SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3281SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: PIP/1571
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the PIP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.~
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Western blot testing of human 1) pancreas and 2) HepG2 lysate with PIP antib
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IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.~
Western blot testing of human 1) pancreas and 2) HepG2 lysate with PIP antib

Documents

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Titering of the PIP antibody may be required for optimal performance.
Description:
It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15), also called Prolactin Inducible Protein, or PIP. It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. PIP is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A human partial recombinant protein corresponding to amino acids 41-146 was used as the immunogen for the PIP antibody.
Limitation:
This PIP antibody is available for research use only.
Localization:
Cytoplasmic (Golgi bodies), secreted
Purity:
Protein G affinity chromatography
Uniprot #:
P12273