PDCD1 Antibody / PD-1 / PD1

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the PD-1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

PDCD-1 (programmed cell death-1 protein), also called PD-1 and CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant full-length human protein was used as the immunogen for the PD-1 antibody.

This PD-1 antibody is available for research use only.

Cell surface & cytoplasmic

Protein G affinity chromatography

Q15116