CD31 Antibody / PECAM-1

NSJ Bioreagents
Product Code: NSJ-V7005SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7005SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 k
Antibody Clonality: Monoclonal
Antibody Clone: PCM25-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
CD31 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

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FACS testing of Jurkat cells with an isotype control (green) and CD31 antibody (red).~
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IHC testing of FFPE colon carcinoma and CD31 antibody (clone PCM25-1). Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA

FACS testing of Jurkat cells with an isotype control (green) and CD31 antibody (red).~
IHC testing of FFPE colon carcinoma and CD31 antibody (clone PCM25-1). Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA

Documents

Further Information

Application Details:
Western blot: 1-2 ug/ml,FACS: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
Application Note:
Titering of the CD31 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
5175
Immunogen:
Recombinant human protein was used as the immunogen for this CD31 antibody.
Limitation:
This CD31 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography