Prohibitin Antibody / PHB

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NSJ Bioreagents
Product Code: NSJ-V7598IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7598IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: PBTN-1
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the PHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human liver tissue with PHB antibody (clone PBTN-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human liver tissue with PHB antibody (clone PBTN-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The stated application concentrations are suggested starting points. Titration of the PHB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Ant-iproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. [RefSeq]
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.
Limitation:
This PHB antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P35232