OX40 Antibody / CD134

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NSJ Bioreagents
Product Code: NSJ-V7534IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7534IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: OX40/3108
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the OX40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human tonsil tissue with OX40 antibody (clone OX40/3108). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human renal cell carcinoma with OX40 antibody (clone OX40/3108). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free OX40 antibody (clone OX40/3108) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with OX40 antibody (clone OX40/3108). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma with OX40 antibody (clone OX40/3108). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free OX40 antibody (clone OX40/3108) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the OX40 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This MAb recognizes a protein of 43kDa, identified as OX40, which is also known as CD134. OX40 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. It is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. It functions as a T cell co-stimulatory receptor when bound by OX40 Ligand / TNFSF4 that is expressed by antigen presenting cells. OX40 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells. OX40 is upregulated at the sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
A portion of amino acids 95-205 from the human protein was used as the immunogen for the OX40 antibody.
Limitation:
This OX40 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P43489