Ornithine Decarboxylase Antibody / ODC1

NSJ Bioreagents
Product Code: NSJ-V7029SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7029SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: ORN255-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the ODC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of ODC antibody and placenta lysate. Predicted molecular weight ~51 kDa.~
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IHC testing of FFPE human prostate carcinoma and ODC antibody (clone ORN255-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM

Western blot testing of ODC antibody and placenta lysate. Predicted molecular weight ~51 kDa.~
IHC testing of FFPE human prostate carcinoma and ODC antibody (clone ORN255-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the ODC antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Ornithine Decarboxylase (ODC) is the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [RefSeq]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
4953
Immunogen:
Recombinant human protein was used as the immunogen for this ODC antibody.
Limitation:
This ODC antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography