CD3 epsilon Antibody

Flow cytometry: 1-2ug/million cells,Immunohistochemistry (acetone fixed frozen sections),Immunofluorescence: 1-2ug/ml,Functional Studies (order BSA/azide-free format)

Optimal dilution of the antibody should be determined by the researcher.

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Human PBMC were used as the immunogen for the CD3e antibody.

This CD3e antibody is available for research use only.

Cell surface, cytoplasmic

Protein G affinity chromatography

P07766