Nuclear Antigen Antibody

NSJ Bioreagents
Product Code: NSJ-V7192IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7192IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: NUCM1-1
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Nuclear Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 2
IHC testing of FFPE rat lung with Nuclear Antigen antibody (clone NUCM1-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.~
2 / 2
IHC

IHC testing of FFPE rat lung with Nuclear Antigen antibody (clone NUCM1-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.~
IHC

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Titering of the Nuclear Antigen antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
Limitation:
This Nuclear Antigen antibody is available for research use only.
Localization:
Nuclei
Purity:
Protein G affinity chromatography