Human Nucleolar Antigen Antibody

NSJ Bioreagents
Product Code: NSJ-V2358CF488
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2358CF488-100T100 Tests£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: NM95
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the Human Nucleolar Antigen antibody at 2-8oC protected from light.

Images

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Intracellular FACS testing of human 293T cells with Human Nucleolar Antigen antibody (green), isotype control (black) and no primary (gray).~
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Immunofluorescent staining of FFPE colon carcinoma with Human Nucleolar Antigen antibod

Intracellular FACS testing of human 293T cells with Human Nucleolar Antigen antibody (green), isotype control (black) and no primary (gray).~
Immunofluorescent staining of FFPE colon carcinoma with Human Nucleolar Antigen antibod

Documents

Further Information

Application Details:
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the Human Nucleolar Antigen antibody should be determined by the researcher.
Description:
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Format:
CF488 Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Nuclei of myeloid leukemia biopsy cells were used as the immunogen for the Human Nucleolar Antigen antibody.
Limitation:
This Human Nucleolar Antigen antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography