NSJ Bioreagents

CD63 Antibody / LAMP-3

Product Code:
 
NSJ-V2070IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NKI/C3
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of human melanoma stained with CD63 antibody (NKI/C3).~
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IHC testing of mouse spleen stained with CD63 antibody (NKI/C3).

IHC testing of human melanoma stained with CD63 antibody (NKI/C3).~
IHC testing of mouse spleen stained with CD63 antibody (NKI/C3).

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NSJ-V2070IHC-7ML7 ml£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
967
Immunogen:
The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this CD63 antibody.
Limitation:
This CD63 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G purified antibody

Documents