Neurofilament Antibody (-Heavy) / NF-H / NEFH

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NSJ Bioreagents
Product Code: NSJ-V7413IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7413IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: NFHP-1R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant NF-H antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human cerebellum with recombinant NF-H antibody (clone NFHP-1R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human cerebellum with recombinant NF-H antibody (clone NFHP-1R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H).
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant NF-H antibody.
Limitation:
This recombinant NF-H antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A affinity
Uniprot #:
P12036