c-Myc Antibody

NSJ Bioreagents
Product Code: NSJ-V2746IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2746IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: MYC909
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the c-Myc antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with c-Myc antibody (MYC909).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with c-Myc antibody (MYC909).

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The stated application concentrations are suggested starting amounts. Optimal dilution of the c-Myc antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant human protein was used as the immunogen for the c-Myc antibody.
Limitation:
This c-Myc antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P01106