MUC1 Antibody / Mucin-1
| Code | Size | Price |
|---|
| NSJ-V2370IHC-7ML | 7 ml | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: MUC1/967
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Epithelial marker antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.
1. FFPE staining REQUIRES boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This Epithelial Membrane Antigen / EMA antibody, also called MUC1 and Mucin-1, recognizes the full-length protein in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this mAb is APDTR in the VNTR region. It reacts with the core peptide of the EMA protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the mAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This EMA antibody reacts with both normal and malignant epithelia of various tissues including breast and colon.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
4582
Immunogen:
Human milk fat globule membranes were used as the immunogen for this Epithelial Marker Antigen antibody.
Limitation:
This Epithelial Marker Antigen antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein G affinity chromatography