Neurofilament Antibody (-Light) / NF-L / NEFL

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NSJ Bioreagents
Product Code: NSJ-V7469IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7469IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: MSI27-2R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant NF-L antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of human cerebellum with recombinant NF-L antibody (clone MSI27-2R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of human cerebellum with recombinant NF-L antibody (clone MSI27-2R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant NF-L antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant NF-L antibody.
Limitation:
This recombinant NF-L antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A affinity chromatography
Uniprot #:
P07196