CD10 Antibody / Neprilysin

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NSJ Bioreagents
Product Code: NSJ-V3878SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3878SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: MME/1892
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human 1) Raji and 2) Ramos cell lysate with CD10 antibody (clone MME/1892). Routinely visualized at ~100 kDa.
2 / 3
Western blot testing of human kidney lysate with CD10 antibody (clone MME/1892). Routinely visualized at ~100 kDa.
3 / 3
SDS-PAGE analysis of purified, BSA-free CD10 antibody (clone MME/1892) as confirmation of integrity and purity.
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Western blot testing of human 1) Raji and 2) Ramos cell lysate with CD10 antibody (clone MME/1892). Routinely visualized at ~100 kDa.
Western blot testing of human kidney lysate with CD10 antibody (clone MME/1892). Routinely visualized at ~100 kDa.
SDS-PAGE analysis of purified, BSA-free CD10 antibody (clone MME/1892) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD10 antibody should be determined by the researcher.
Description:
CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA) and Neprilysin, is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 297-483 from the human protein was used as the immunogen for the CD10 antibody.
Limitation:
This CD10 antibody is available for research use only.
Localization:
Cell surface, Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P08473