MUC2 Antibody / Mucin 2

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the MUC2 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10 for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This mAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant human protein was used as the immunogen for the MUC2 antibody.

This MUC2 antibody is available for research use only.

Cytoplasmic and cell surface

Protein G affinity chromatography

Q02817