Ki67 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3864SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3864SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: MKI67/2462
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human skin stained with Ki67 antibody (MKI67/2462). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 6
IHC testing of FFPE human tonsil stained with Ki67 antibody (MKI67/2462). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 6
Immunofluorescent staining of human HeLa cells with Ki-67 antibody (green, clone MKI67/2462) and Phalloidin (red, membrane stain).
4 / 6
FACS testing of human HeLa cells with Ki-67 antibody (blue, clone MKI67/2462) and isotype control (red). Cells were trypsinized and 2-4% PFA-fixed prior to staining.
5 / 6
SDS-PAGE analysis of purified, BSA-free Ki67 antibody (clone MKI67/2462) as confirmation of integrity and purity.
6 / 6
Western blot testing of human Raji and Ramos cell lysate with Ki67 antibody. Expected molecular weight ~350 kDa.
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IHC testing of FFPE human skin stained with Ki67 antibody (MKI67/2462). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human tonsil stained with Ki67 antibody (MKI67/2462). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human HeLa cells with Ki-67 antibody (green, clone MKI67/2462) and Phalloidin (red, membrane stain).
FACS testing of human HeLa cells with Ki-67 antibody (blue, clone MKI67/2462) and isotype control (red). Cells were trypsinized and 2-4% PFA-fixed prior to staining.
SDS-PAGE analysis of purified, BSA-free Ki67 antibody (clone MKI67/2462) as confirmation of integrity and purity.
Western blot testing of human Raji and Ramos cell lysate with Ki67 antibody. Expected molecular weight ~350 kDa.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the Ki67 antibody should be determined by the researcher.
Description:
Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.
Limitation:
This Ki67 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P46013