Mammaglobin A Antibody / SCGB2A2

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NSJ Bioreagents
Product Code: NSJ-V7436SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7436SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: MGB/2682R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Mammaglobin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Immunofluorescent staining of permeabilized human MCF7 cells with recombinant Mammaglobin antibody (clone MGB/2682R, green) and Reddot nuclear stain (red).
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IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of permeabilized human MCF7 cells with recombinant Mammaglobin antibody (clone MGB/2682R, green) and Reddot nuclear stain (red).

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Mammaglobin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Mammaglobin, also called Secretoglobin family 2A member 2, or SCGB2A2, is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant human protein was used as the immunogen for the recombinant Mammaglobin antibody.
Limitation:
This recombinant Mammaglobin antibody is available for research use only.
Localization:
Cytoplasmic, cell surface, nuclear
Purity:
Protein A affinity chromatography
Uniprot #:
Q13296