MBP Antibody / Myelin Basic Protein
| Code | Size | Price |
|---|
| NSJ-V8680SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: MBP/4276
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the Myelin Basic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
Description:
Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. Phosphorylation been shown to decrease its ability to aggregate lipid vesicles and consequently destabilising the compact structure ofmyelin, a destruction that has been observed in demyelinating diseases such as Multiple Sclerosis. MBP/4276 is a useful detector of phosphorylated MBP by binding to Thr98 of human MBP in the phosphorylated state.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A synthetic peptide corresponding to the amino acids surrounding phosphorylated threonine 98 were used as the immunogen for the Myelin Basic Protein antibody.
Limitation:
This Myelin Basic Protein antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein G affinity chromatography
Uniprot #:
P02686