MAML2 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V3532SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3532SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: MAML2/1302
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the MAML2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC testing of FFPE human pancreas tissue with MAML2 antibody (clone MAML2/1302). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IHC testing of FFPE human colon carcinoma with MAML2 antibody (clone MAML2/1302). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE Analysis of Purified, BSA-Free MAML2 Antibody (clone MAML2/1302). Confirmation of Integrity and Purity of the Antibody.
❮❯

IHC testing of FFPE human pancreas tissue with MAML2 antibody (clone MAML2/1302). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human colon carcinoma with MAML2 antibody (clone MAML2/1302). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free MAML2 Antibody (clone MAML2/1302). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML2 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Mastermind-like 2, also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, it interacts with the ankyrin repeats of the Notch proteins (1-4). This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jkappa; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
84441
Immunogen:
A human recombinant full length protein was used as the immunogen for this MAML2 antibody.
Limitation:
This MAML2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography