Melanoma Cell Marker Antibody Cocktail (anti-MART-1 + gp100 + Tyrosinase)
| Code | Size | Price |
|---|
| NSJ-V3157SAF-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 + IgG2a + IgG2b + IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: M2-7C10 + M2-9E3 + HMB45 + T311
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the Melanoma Cell Marker antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the Melanoma Cell Marker antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant MART-1 protein (M2-7C10 & M2-9E3), recombinant tyrosinase protein (T311) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogens for this Melanoma Cell Marker antibody cocktail.
Limitation:
This Melanoma Cell Marker antibody cocktail is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
Q16655 (Melan-A), P14679 (Tyrosinase), P40967 (gp100)