MART-1 / Melan-A Antibody

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NSJ Bioreagents
Product Code: NSJ-V2116IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2116IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b lambda
Antibody Clonality: Monoclonal
Antibody Clone: M2-7C10
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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IHC testing of FFPE human skin stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human skin stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
2315
Immunogen:
Recombinant human MART-1 protein was used as the immunogen for this antibody.
Limitation:
This MART-1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography