LMO2 Antibody / Rhombotin 2

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NSJ Bioreagents
Product Code: NSJ-V7925SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7925SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: LMO2/1971
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the LMO2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Western blot testing of human Ramos cell lysate with LMO2 antibody. Predicted molecular weight ~25 kDa.
2 / 5
Flow cytometry testing of permeabilized human K562 cells with LMO2 antibody; Red=isotype control, Blue= LMO2 antibody.
3 / 5
IHC staining of FFPE human follicular lymphoma with LMO2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human pancreas with LMO2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
5 / 5
SDS-PAGE analysis of purified, BSA-free LMO2 antibody as confirmation of integrity and purity.
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Western blot testing of human Ramos cell lysate with LMO2 antibody. Predicted molecular weight ~25 kDa.
Flow cytometry testing of permeabilized human K562 cells with LMO2 antibody; Red=isotype control, Blue= LMO2 antibody.
IHC staining of FFPE human follicular lymphoma with LMO2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human pancreas with LMO2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free LMO2 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. It has a particular function in normal and lymphatic endothelial cells involving the regulation of angiogenesis and lymph-angiogenesis. Immunohistochemical studies have also demonstrated expression of LMO2 in both normal germinal center B-cells and germinal center-derived B-cell lymphomas, including follicular lymphoma and diffuse large B-cell lymphoma.The use of anti-LMO2 is valuable as a tool in the identification of lymphomas of B-cell origin. LMO2 is useful in differentiating follicular lymphoma (LMO2+) from nodal marginal zone lymphoma (LM02-). It also is positive in Hodgkin s and Burkitt s lymphomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human partial protein (amino acids 23-140) was used as the immunogen for the LMO2 antibody.
Limitation:
This LMO2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P25791