CD63 Antibody / LAMP3

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NSJ Bioreagents
Product Code: NSJ-V3028SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3028SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: LAMP3/529
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the LAMP-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry testing of PFA-fixed human U-87 MG cells with LAMP-3 antibody (clone LAMP3/529); Red=isotype control, Blue= LAMP-3 antibody.~
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SDS-PAGE analysis of purified, BSA-free LAMP-3 antibody as confirmation of integrity and purity.
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Flow cytometry testing of PFA-fixed human U-87 MG cells with LAMP-3 antibody (clone LAMP3/529); Red=isotype control, Blue= LAMP-3 antibody.~
SDS-PAGE analysis of purified, BSA-free LAMP-3 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details:
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 0.5-1ug/ml
Application Note:
Optimal dilution of the LAMP-3 antibody should be determined by the researcher.
Description:
This mAb recognizes protein of 26kDa-60kDa, which is identified as CD63/LAMP-3. Its epitope is different from that of mAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63/LAMP-3 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. It is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP-3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the LAMP-3 antibody.
Limitation:
This LAMP-3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P08962