CD20 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7645SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7645SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: L26
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

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IHC staining of FFPE human tonsil tissue with CD20 antibody (clone L26). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human lymphoma with CD20 antibody (clone L26). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 6
Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone L26) and Reddot nuclear stain (red).
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Flow cytometry testing of human Raji cells with CD20 antibody (clone L26); Red=isotype control, Blue= CD20 antibody.
5 / 6
Western blot testing of human Raji lysate with CD20 antibody (clone L26). Predicted molecular weight ~33 kDa.
6 / 6
SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone L26) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with CD20 antibody (clone L26). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma with CD20 antibody (clone L26). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone L26) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with CD20 antibody (clone L26); Red=isotype control, Blue= CD20 antibody.
Western blot testing of human Raji lysate with CD20 antibody (clone L26). Predicted molecular weight ~33 kDa.
SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone L26) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human tonsil B cells were used as the immunogen for this CD20 antibody.
Limitation:
This CD20 antibody is available for research use only.
Localization:
Predominantly cell surface with some cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P11836