Ku70 + Ku80 Antibody
| Code | Size | Price |
|---|
| NSJ-V2128PE-100T | 100 Tests | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: KU729
Regulatory Status: RUO
Target Species:
- Human
- Non-Human Primates
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
Storage:
Store the Ku70 + Ku80 antibody at 2-8oC. Conjugate is light sensitive store in the dark.
Documents
Further Information
Application Details:
Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
Description:
This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Format:
PE Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
2547
Immunogen:
Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
Limitation:
This Ku70 + Ku80 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography